Ultrasonic Bioactive Extraction

Product Description:

Natural products often have active components that are intracellular, requiring cell disruption for extraction. Conventional extraction methods include decoction, maceration, percolation, reflux extraction, and steam distillation. However, these methods often fail to achieve ideal cell disruption, leading to drawbacks such as low extraction yields, high solvent consumption, significant energy usage, and long processing times.

Ultrasonic bioactive extraction technology utilises the intense vibration, high acceleration, strong cavitation effects, and powerful agitation of ultrasound to shorten the time it takes for active ingredients to enter the solvent. This accelerates the extraction process, increases the yield, and effectively prevents the degradation of active components caused by high temperatures.

In an experiment on soybean isoflavone extraction, using 70% ethanol with a solid-to-liquid ratio of 1:6 at 50°C for 5 hours yielded 4.07 mg/g of isoflavones. Under the same conditions but with ultrasonic assistance, the yield increased to 4.23 mg/g, an improvement of 3.93%. Therefore, with optimised extraction conditions, ultrasonic assistance can enhance extraction efficiency. Furthermore, the ultrasonic extraction process showed no significant impact on the physiological activity of the soybean isoflavones, such as their antioxidant capacity.

In practice, ultrasonic bioactive extraction technology is widely used across various fields, including traditional Chinese medicine preparations, the nutraceutical industry, and chemical analysis.

Product Parameter:

Features of Ultrasonic Bioactive Extraction Machine:

1. Features a 4.3-inch intelligent touchscreen display;

2. Equipped with an automatic frequency tracking function;

3. Probe made from high-quality industrial-grade titanium alloy;

4. Continuously adjustable power output;

5. Offers excellent corrosion resistance and high heat tolerance;

6. Low-noise metal casing with high-quality soundproofing material;

7. Available with a variety of probe sizes;

8. Capable of creating and storing up to 20 operating programs.

FAQ:

What solvents are used in ultrasonic bioactive extraction?

The extraction of bioactive compounds (such as flavonoids, alkaloids, and polysaccharides) using ultrasound typically employs solvents like alcohols, water, ketones, or their mixtures. Commonly used solvents include ethanol (40%-80%), methanol, acetone, ethyl acetate, and water. The selection principle is based on the polarity of the target compound, environmental considerations, and cost. Aqueous ethanol is the most frequently used solvent for pharmaceutical extraction due to its low toxicity and high efficiency.

What temperature is suitable for ultrasonic bioactive extraction?

The optimal temperature for ultrasonic bioactive extraction typically ranges from 40°C to 60°C. Within this range, a moderate temperature increase can reduce solvent viscosity, enhance molecular movement, and promote the diffusion of bioactive compounds, while avoiding the inactivation or degradation of heat-sensitive components caused by excessive heat. For heat-sensitive substances such as polyphenols, flavonoids, and polysaccharides, a lower temperature range of 40°C to 50°C is generally recommended to preserve their activity. During the extraction process, temperatures should not exceed 60°C, as higher temperatures can lead to the decomposition of most bioactive constituents and may also cause solvent evaporation.

How does the extraction rate of an ultrasonic extractor vary?
The extraction rate of an ultrasonic extractor differs depending on the active compound being extracted. Taking nicotine extraction from tobacco as an example: using 40% methanol as the extraction solvent with a solid-to-liquid ratio of 1:20, an extraction time of 30 minutes, and an ultrasonic temperature of 150°C, the nicotine extraction rate can reach approximately 7%.

When 0.4% NaOH is used as the extraction solvent with a solid-to-liquid ratio of 1:40, an ultrasonic extraction time of 4 hours at room temperature, the nicotine extraction rate is 2.12%.

Using 78% ethanol as the solvent with a solid-to-liquid ratio of 1:5, an extraction time of 30 minutes, and an ultrasonic temperature of 52°C, the nicotine extraction yield is 23.1 mg/mL.

With 95% ethanol at pH 4 as the solvent, a solid-to-liquid ratio of 1:15, and an ultrasonic temperature of 80°C, performing two extractions of 45 minutes each results in a nicotine extraction rate of 95.42%. Comparative analysis shows that this method is significantly more effective than heat reflux extraction, which achieves only 84.79%.

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