Ultrasonic Cell Crusher

Working principle of ultrasonic cell crusher

The propagation of ultrasonic waves in the medium can cause the particles in the medium to vibrate in their propagation space, thereby enhancing the diffusion and propagation of the medium. This is the mechanical fragmentation effect of ultrasonic waves. When ultrasonic waves propagate, radiation pressure is generated. This pressure has a strong destructive effect and can deform cell tissues and denature plant proteins; at the same time, it can provide different accelerations to the medium and suspension, and the speed of the medium is much faster than the speed of the suspension molecules. The friction between the two causes the biomolecules to disaggregate, allowing the active ingredients in the cell wall to dissolve into the solvent more quickly.

Specification of Ultrasonic Cell Crusher

Other applications：

1. Fragments: Escherichia coli, bacteria, DNA strand cutting of animal and plant cells, yeast/fermentation bacterial fragments, intracellular organelles

2. Extraction: extract components from cells, isolate and recover viruses, soil microorganisms, and immune deposition of chromatin

FAQ: 

Q:  What is the difference between an ultrasonic cleaning instrument and a cell disruptor?

The main principles of ultrasonic cleaning instruments and ultrasonic cell breakers are the same. The main difference is that the ultrasonic cell crusher concentrates all the energy on the probe, while the energy of the ultrasonic cleaner is evenly distributed at the bottom of the cleaning tank. Therefore, Ultrasonic cleaners tend to degas and clean, while ultrasonic cell disrupters tend to disrupt, emulsify, and mix cells.

Precautions

1. The instrument should be placed in a clean and dry place, and should not be placed in a place with moisture, high temperature, dust or corrosive gas.

2. The horn selection switch on the rear panel of the instrument should be at the corresponding position of the horn, and the new horn should correspond to the selection switch.

3. The selection of container capacity and shape should be coordinated with the size of the sample. The use of slender containers has the best crushing effect.

4. It is recommended to crush multiple times in a short time, the ultrasonic time should be within 5 seconds, and the interval should be less than 5 seconds.

5. The depth of the horn being wiped into the liquid level is between 10-15mm, and the distance between the end and the bottom of the container is greater than 30 mm. It is strictly forbidden to turn on the machine without liquid inserted into the end.

6. The transducer must be firmly fixed on the bracket to prevent it from sliding down from the pole. Do not hit the end of the horn to prevent deformation or damage.

7. When the crushing volume is less than 5ml, use a Φ2 horn. At this time, the insertion depth of the horn is about 1 mm, and the distance between the end and the bottom should not be less than 5 mm. The best ultrasonic time is between 0.5-1 seconds.

8. Rotate the power adjustment button to adjust the power. The power should be turned smaller to avoid overloading the horn and causing it to break. Never exceed the maximum power shown in the right window.

9. When an external signal fails or a protection indication occurs, the protection indicator light lights up and the instrument stops working, prompting the staff to eliminate the fault. After the fault is eliminated, press the protection reset button to resume normal operation.

10. Please fill in the ultrasonic cell crusher usage record in time after each use.